Trio-Smart SIBO Breath Test

Test For All 3 Types of SIBO
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Nutrition Consultation Package: Gemelli Biotech

The trio-smart breath test tests for 3 types of SIBO

The trio-smart breath test is applicable to patients who experience bloating, gas, abdominal pain, constipation and/or diarrhea.

Trio-smart measures the levels of hydrogen, methane, and hydrogen sulfide in your breath. Elevated levels of gases can indicate small intestinal bacterial overgrowth (SIBO), intestinal methanogenic overgrowth (IMO), and/or excess hydrogen sulfide.

How does the trio-smart breath test work?

trio-smart is a doctor-ordered breath test that uses an at-home sample collection kit. Using the kit, you collect your samples by breathing into 9 collection bags over a 2-hour period. The first bag is considered a “baseline” sample, and the remaining samples are collected after you drink a sugar substrate drink.

You then send your samples to our CLIA-certified lab (Prepaid shipping materials are included.), and the test results are reported to your doctor within a week.

A step-by-step sample collection instructional video is available here.

What does the trio-smart breath test measure?

The trio-smart breath test measures the three primary fermented gases in your microbiome by way of your breath before and after a sugar substrate drink is consumer. The gases measured are hydrogen, methane, and hydrogen sulfide.

Hydrogen is produced when certain bacteria consume sugars.

  • Hydrogen levels are considered abnormal when they rise greater than ≥20 ppm (parts per million) from the baseline within 90 minutes.

Methane is produced when archaea (single-celled organisms similar to bacteria) consume hydrogen.

  • Methane levels are considered abnormal if they reach ≥10 ppm (parts per million) at any point during the breath test.

Hydrogen sulfide is produced when sulfate-reducing organisms consume hydrogen.

  • Hydrogen sulfide levels are considered abnormal if they reach ≥3ppm (parts per million) at any point during the breath test.

Data from the trio-smart breath test can help guide treatment and is the fist step towards feeling better. Learn more:


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